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鱼皮质醇Elisa试剂盒

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上传时间：2015/10/24 16:32:21

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上传者：北京绿源大德生物科技有限公司

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鱼皮质醇elisa试剂盒

Generic Name：Fish Cortisol ELISA Kit.

Purpose

This kit allows for the determination of Cortisol concentrations in Fish serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Fish Cortisol level in the sample，use Purified Fish Cortisol antibody to coat microtiter plate wells, make solid-phase antibody, then add Cortisol to wells, Combined Cortisol antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Cortisol in the samples is then determined by comparing the O.D. of the samples to the standard curve.