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His标签抗体
点击次数：525
价格：￥1050
更新日期：2025/11/7 5:34:47
生 产 地：中国大陆
产品型号：ICP0850
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相关标签：His,标签,抗体
详细内容
| Product Description | This high affinity His-tag antibody is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH ａnd affinity-purified with the HHHHHH peptide on agarose. The antibody is useful for detection, affinity isolation, enrichment and localization of His-tag proteins. | |  Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate ａnd enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 µL of His-tag protein in lysate (original cell lysate) 3: 10 µL of His-tag protein in lysate (after IP) 4: 10 µL of the His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag (ICP0850) from 2 mL of the original lysate |
| Species | Rabbit | ||
| Formulation | 500 µg/mL in PBS, 50% glycerol | ||
| Immunogen | Polyhistidine peptide (HHHHHH) KLH conjugates | ||
| Purification | Antigen-specific affinity chromatography | ||
| Specificity | The antibody recognizes His-tag proteins. | ||
| Applications | ELISA (0.25-1 µg/mL); IP ( 1-10 µg, based on the sample size) ; WB (0.25-1.0 µg/mL); IHC (5 µg/mL) | ||
| Scientific Description | Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix. | ||
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. |
His,标签,抗体
