Catalog # Product Description	ICP0388-5mg In order to increase the efficeincy ａnd capacity of the affinity enrichment, higher density of the acetylated lysine antibody is immobilized to beaded agarose. The product could be utilized as an affinity matrix for rapid isolation ａnd purification of the species of proteins or peptide with acetyl lysine residues.	The acetylated protein Signal as determinated by ELISA titration (OD50 titer). Acetlated Proteins were eluted from 1 mL the rabbit anti-acetyllysine Agarose (ICP0388) ａnd immobilized on microrplate followed by detection with mouse anti-Acetylated lysine HRP. Rabbitt anti-Biotin Agarose (1 mL) was utilized as negative control. The maximum binding of acetylated BSA with ICP0388-5MG. -50 µl of ICP0388-5MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min. - After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl -5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE ａnd blot wih monoclonal anti-acetylated lysine (ICP0390) -ECL exposure 3 seconds
Formulation	0.5 mL beaded agarose suspended in 1 mL slurry
Antibody Immobilized	10 mg/mL antibody is covalently linked through amide bonds with NHS activated-SMCC then linked to thiolated agarose beads via thiol ether bonds.
Specificity	This antibody affinity matrix selectively captures the peptides ａnd proteins with acetylated lysine residues (N-epsilon). There is no cross creation with methylated proteins or mono- ａnd demethylated proteins.
Binding Capacity	Approximately 0.5-1 mg of acetylated histone/ml.
Applications	Rapid isolation ａnd purification of peptides or proteins with acetylated lysine residues from cell lysate or protease-digested mixtures.
Scientific Description	Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation ａnd identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient ａnd rapid methods to enrich ａnd purify the acetylated species for further MS/MS identification.
Storage & Stability	Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Do not aliquot ａnd shake thoroughly before use.