磷酸化酪氨酸抗体

Product Description:Affinity purified rabbit polyclonal anti-phosphotyrosine antibody (anti-pY) was developed using peptidyl-phosphotyrosine analog-KLH conjugates as immunogen. The phosphotyrosine specific antibodies were affinity purified with phoshotyrosine immobilized on agarose. This antibody could be utilized for immunoprecipitation of tyrosine-phosphorylated immunoblotting tyrosine-phosphorylated proteins, ELISA quantitation of protein tyrosine phosphorylation, ａnd non-radioactive tyrosine kinase assay.

Species:Rabbit

Formulation:250 µg/mL in PBS, pH7, 0.01% NaN₃.

Storage & Stability:Store product at –20 ℃. Three years from date of shipping.

Immunogen:Lodo acetyl phospho tyramine KLH conjugates.

Purification:Affinity purified with phosphotyorsine on Agarose.

Specificity:The antibody recognizes proteins ａnd peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine ａnd threonine.

Tested:Human breast cancer tissue.

Application(s):ELISA(1:1000), WB(1:250), IP(10 µg/500 µg protein sample), IHC(1:50), non-radioactive tyrosine kinase assay.

Scientific Description:Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phosphotyrosine which is considered as a key step in the regulation of enzymatic activities such as signal transduction.

References:Hoffhines, A. J. et al. J. Bio. Chem. 2006, 281, 37877-37887.