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技术文章
Anti-Factor VIII抗体,凝血因子8抗体说明书
点击次数：3615 发布时间：2016/9/5 10:36:24
Anti-Factor VIII抗体,凝血因子8抗体说明书
Rabbit Anti-factor VIII antibody
Supplier: Shanghai Yuan Mu Biotechnology Co., Ltd.
Quantity size : 0.1ml (1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol)
Background: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 ａnd phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma ａnd associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
Specificity :
– Anti-factor VIII is a Rabbit Polyclonal antibody unconjugated
– specific for factor VIII of Human, Mouse, Rat, Dog, Rabbit
– use for western blotting,elisa,immunoprecipitation ａnd immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 267kDa
Application :
– ELISA=1:500-1000
– IHC-P=1:100-500 IHC-F=1:100-500
– ICC=1:100-500 IF=1:100-500
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20 for one year. ℃ Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20 . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ℃ antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.
储存：伟大的实习20为一年。℃，避免重复冻结/解冻周期。《lyophilized抗体在冰室温度稳定在至少一个月和一年时，更大的比kept -20。当滚筒在0.01M PBS，pH值7.4不育系或diluent℃冰的抗体，抗体稳定的至少六周的实习2～4℃
重要说明：本品为supplied冰用于研究使用，只有规范，标注用于人，治疗或诊断的应用。
原创作者：上海远慕生物科技有限公司
