上海笃玛生物科技有限公司
第12年
当前位置：阿仪网 > 上海笃玛生物科技有限公司 > 技术文献 > Gab1 Antibody Catalog Number：OM233623
发布时间：2024/9/14 13:59:34关注度：2738
| Product Name | Gab1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1AT1979 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB~~1:1000: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 76616 Da |
| Function | Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) ａnd the insulin receptor (INSR) |
| Tissue Specificity | This Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1. |
| Entrez Gene | 2549 |
|---|
Wild-type (+/+) ａnd Gab1-/- (-/-) cells were heat shocked (HS) at 42掳C for 1 h or irradiated with UV-B light (400 J/m2) ａnd then incubated at 37? for 1 h. Cell lysates were prepared ａnd subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE ａnd immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539)
| Application Notes | WB~~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS. |
原创作者：上海笃玛生物科技有限公司
相关信息
推荐产品
ELISA试剂盒，动物血清，生化试剂盒，标准品，对照品，试剂，耗材，抗体，培养基，代理进口品牌，实验代测服务
在线咨询5分钟应答
