AT5G11260 HY5小鼠单克隆抗体

Description:

Mouse monoclonal antibody

Background:

HY5 is a basic leucine zipper (bZIP) transcription factor with nuclear localization. It is involved in light-regulated transcriptional activation of G-box-containing promoters. HY5 plays a role in anthocyanin accumulation in far-red light ａnd blue light, but not in red light or in the dark.

Synonyms:

HY5, ELONGATED HYPOCOTYL 5, REVERSAL OF THE DET PHENOTYPE 5, TED 5

Immunogen:

Recombinant protein of HY5 derived from Arabidopsis thaliana AT5G11260.

Form:

Lyophilized

Quantity:

150 µg

Purification:

Protein A purified

Reconstitution:

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

Stability &
Storage:

Use a manual defrost freezer ａnd avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to ⁻⁷0℃ as supplied.
6 months, -20 to ⁻⁷0℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Applications:

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

Expected Results:

18 kDa

Confirmed Reactivity:

Arabidopsis thaliana

Predicted Reactivity:

For more species homologues information, please contact tech support at tech@phytoab.com.

Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006)
Detection: using chemiluminescence substrate ａnd image were captured with CCD camera.