MS培养基 植物培养基 （不含维生素）

MS植物培养基

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium ａnd originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM ａnd higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich ａnd -saline -medium ａnd may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier ａnd Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.

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