FST Human

Catalogue Number CYT-232
Synonyms FST, FS, Activin-binding protein.
Introduction Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 ａnd FST344 containing 317 ａnd 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage ａnd association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS ａnd follistatin. Follistatin binds directly to activin ａnd functions as an activin antagonist. specific inhibitor of the biosynthesis ａnd secretion of pituitary follicle stimulating hormone (fsh).
Description Follistatin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids ａnd having a total molecular mass of 31.5kDa.
The FST is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Solubility It is recommended to reconstitute the lyophilized Follistatin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FST should be stored at 4°C between 2⁻⁷ days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined ａnd was found to be Gly-Asn-Cys-Trp-Leu.
Biological Activity The activity is determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells. The expected ED50 is 100-400ng/ml corresponding to a Specific Activity of 2,500-10,000IU/mg in the presence of 7.5ng/ml Activin A.
Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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