EGF Human

Epidermal Growth Factor Human Recombinant Shipping:SynonymsUrogastrone, URG, EGF.IntroductionEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo ａnd is a potent mitogenic factor for a variety of cultured cells of both ectodermal ａnd mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal ａnd epithelial tissues in vivo ａnd in vitro ａnd of some fibroblasts in cell culture.DescriptionEpidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids ａnd having a molecular mass of 6222 Dalton.
The EGF is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized （freeze-dried） powder.FormulationThe protein was lyophilized from a concentrated （1mg/ml） solution containing PBS pH⁻⁷.4.SolubilityIt is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2⁻⁷ days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.PurityGreater than 98.0% as determined by:
（a） Analysis by SEC-HPLC.
（b） Analysis by SDS-PAGE.Amino acid sequenceNSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.Biological ActivityThe ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells （measured by 3H-thymidine uptake） is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg.
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