5甲基胞嘧啶（5-mC）单克隆抗体

Product Overview

Description
Mouse monoclonal antibody to 5-methylcytosine （5-mC）, clone 33D3, MeDIP/ChIP-grade

Concentration
1 mg/ml

Purification
Protein A

Immunogen
Ovalbumin-conjugated 5-methylcytosine （5-mC）

Isotype
IgG1

Specificity
Modified base 5-methylcytosine （5-mC）, a broad range of species

Formulation
Purified IgG in 10 mM phosphate buffer, 150 mM NaCl, pH 7.4

Storage
4°C, stable for 6 months from the date of shipment. For long-term storage, aliquot ａnd store at -20°C. Avoid repeated freezing ａnd thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternate Names
5-methylcytidine, anti-5-methylcytidine, anti-5-methylcytosine, anti-5mC, anti-5-mC, anti-5meC, anti-5-meC, 5mC, 5meC, 5-meC, 5＇-methyl-2＇-deoxycytidine, 5MedCyd

Application & Suggested Dilutions*
Dot Blot: 1:1000-1:2000; Immunohistochemistry: 1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:1000-1:2000; MeDIP: 0.5-1 µg/reaction

*The end user is responsible for determining optimal working dilutions.

Fig. 1. Methylated DNA successfully captured by anti-5-methylcytosine clone 33D3 （Cat #A-1014） during MeDIP.


Fig. 2. Immunofluorescence staining with anti-5-methylcytosine clone 33D3 （Cat #A-1014）.

Fig. 3. Synthesized oligos containing different numbers of 5-methylcytosines were captured with the Clone 33D3 anti-5-methylcytosine antibody （Cat No. A-1014） ａnd then colorimetrically detected. The results show that the oligos containing as few as two 5-mCs can still be captured ａnd oligos with four or more 5-mCs can be fully captured by the antibody.

Fig. 4. Synthesized oligos containing different numbers of 5-methylcytosines were captured with anti-5-methylcytosine antibodies from various companies ａnd then colorimetrically detected for comparison. Results show Epigentek＇s 5-mC antibody has the highest sensitivity ａnd specificity in capturing methylated DNA fragments.

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