新品：高精度cDNA合成试剂盒-Epigentek

 EpiNext高精度cDNA合成试剂盒（EpiNext Hi-Fi cDNA Synthesis Kit）

Epigentek货号：P-9004-20(20个样品)， 启维益成货号：E176973

高精度cDNA合成试剂盒（EpiNext Hi-Fi cDNA Synthesis Kit）使用亚硫酸盐修饰的RNA和RN段合成DNA，含整套优化的试剂。合成的cDNA可用于实时定量PCR，RNA/cDNA测序等下游实验。为使用亚硫酸盐修饰的RNA合成DNA而优化；也适用于用总RNA和GC含量高的RNA合成cDNA；合成的DNA可用于PCR和cDNA文库构建；试剂盒包含的反转录酶的RNA酶活性极低，热稳定性高，不被rRNA和tRNA抑制，因而反转录的准确度极高并且促进全长cDNA的合成。

适用于不同组织和细胞的RNA样品，起始RNA量0.1到2ng，最佳RNA量200-500ng。

Product Overview
The EpiNext™ Hi-Fi cDNA Synthesis Kit is a complete set of optimized reagents to synthesize a DNA copy specifically using bisulfite-converted RNA ａnd enriched RNA fragments.
.Optimized to synthesize DNA copies from bisulfite-converted RNA
.Also suitable for cDNA synthesis from total RNA
.Synthesized DNA is fully compatible with PCR ａnd cDNA library construction
.Included RT enzyme has low instrinsic RNase activity to deliver extremely high reverse transcription accuracy ａnd promotes full-length cDNA synthesis

Description
Effective ａnd efficient preparation of cDNA from RNA can help ensure reliable data in various downstream applications including real-time qPCR ａnd RNA/cDNA-sequencing. The EpiNext™ Hi-Fi cDNA Synthesis Kit has been optimized ａnd validated for cDNA synthesis from various RNA samples, particularly from bisulfite-converted RNA ａnd enriched RNA fragments. The kit contains all necessary components including a highly sensitive ａnd thermostable recombinant reverse transcriptase which provides enhanced cDNA synthesis efficiency, high fidelity, ａnd a wide range of activity for varying amounts of RNA templates (0.1 ng to 2 µg). Due to its high thermal stability, low RNase activity ａnd resistance to inhibition by rRNA ａnd tRNA, the recombinant RT included in the kit can be used for synthesis of long cDNA products at temperatures from 42°C-60°C using total RNA, ａnd GC-rich templates such as bisulfite converted RNA. A recombinant RNase inhibitor is also included in the kit to prevent the degradation of target RNA due to ribonuclease contamination.

Starting Materials
RNA isolated from various tissue or cell samples can be used as starting material. The amount of RNA for each reaction can be 0.1 ng to 2 µg. For an optimal reaction, the input RNA amount should be 200 ng to 500 ng.

Fig. 1. The EpiNext HiFi cDNA Synthesis Kit mechanism uses both A) oligo-dT ａnd B) random hexamers.

Product Components
5X RT reaction buffer
10 mM dNTP mix*
0.1M DTT*
RNase inhibitor*
Random primer (50 µM)*
RT enzyme mix*
User Guide

*Spin the solution down to the bottom prior to use.