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人羊膜上皮细胞

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更新日期：2026/8/24 23:48:28

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简单介绍：人羊膜上皮细胞
<7110> 人羊膜上皮细胞(HAmEpiC)提取于人羊膜组织，于原代冻存。

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人羊膜上皮细胞（HAEpiC）提取于人羊膜组织，于原代冻存。每管含有细胞数>5×10⁵ cells/ml，此细胞通过Cytokeratin-18, Cytokeratin-19和Vimentin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

推荐培养基:（EpiCM, Cat. No. 4101）

产地：San Diego,US
品牌：ScienCell

储存：液氮

运输：干冰

用途：科研

Human amniotic membrane is composed of an epithelial cell layer, a basement membrane ａnd an avascular matrix. The amniotic epithelial cells （AEC） are formed from epiblasts on the 8th day after fertilization. A probable result of their embryonic origin, AEC lack major histocompatibility complex antigens ａnd have been used for allotranplantation to treat patients with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis ａnd release of acetylcholine ａnd catecholamine as well as expressing mRNA coding for dopamine receptors ａnd dopamine transporter [1]. They express neuronal ａnd glial cell marks ａnd produce basic fibroblast growth factor, hepatocyte growth factor ａnd transforming growth factor-beta [2]. Human AEC has been suggested as an appropriate human cell model for studying dopamine release ａnd uptake processes, receptor signal transduction ａnd exploring newly developed drugs acting at these receptors [3].

HAEpiC from ScienCell Research Laboratories are isolated from human amniotic membrane. HAEpiC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HAEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 ａnd vimentin. HAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HAEpiC are guaranteed to further culture in the condition provided by ScienCell Research Laboratories.

Reference
[1] Sakuragawa, N., Elwan, M. A., Uchida, S., Fujii, T., Kawashima, K. （2001） Non-neuronal neurotransmitters ａnd neurotrophic factors in amniotic epithelial cells: expression ａnd function in humans ａnd monkey. Jpn J Pharmacol. 85（1）:20-3.
[2] Ishii, T., Ohsugi, K., Nakamura, S., Sato, K., Hashimoto, M., Mikoshiba, K., Sakuragawa, N. （1999） Gene expression of oligodendrocyte markers in human amniotic epithelial cells using neural cell-type-specific expression system. Neurosci Lett. 268（3）:131-4.
[3] Elwan, M. A., Ishii, T., Sakuragawa, N. （2003） Characterization of the dopamine transporter gene expression ａnd binding sites in cultured human amniotic epithelial cells. Neurosci Lett. 342（1-2）:61-4.