Anti-SPARC富含半胱氨酸的酸性分泌蛋白抗体

本公司经销SPARC，富含半胱氨酸的酸性分泌蛋白抗体，克隆类型为polyclonal，宿主来源是Rabbit，SPARC富含半胱氨酸的酸性分泌蛋白抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-1133R
英文名称：Anti-SPARC
中文名称：富含半胱氨酸的酸性分泌蛋白抗体
其他名称：名AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic ａnd rich in cysteine; Secreted protein acidic cysteine rich （osteonectin）; Secreted protein acidic cysteine rich; SPRC.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 33kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, dog, pig, cow, hrs, Rb
产品介绍：Secreted protein acidic ａnd rich in cysteine is a number of ways of regulating cell function of extracellular matrix protein, SPARC protein in human tissue ａnd widely distributed in the reconstruction ａnd cancer-related organizations. SPARC in a variety of tumors had high expression. Gene Symbol: SPARC; Osteonectin; BM-40; ON.Function : Appears to regulate cell growth through interactions with the extracellular matrix ａnd cytokines. Binds calcium ａnd copper, several types of collagen, albumin, thrombospondin, PDGF ａnd cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca（2+） with a low affinity ａnd an EF-hand loop that binds a Ca（2+） ion with a high affinity.Subcellular Location : Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.Similarity : Belongs to the SPARC family.Contains 1 EF-hand domain.Contains 1 follistatin-like domain.Contains 1 Kazal-like domain.SPARC是从多方面调节细胞功能的细胞外基质蛋白，SPARC蛋白在人组织中广泛分布与组织重建和肿瘤有关。SPARC在多种肿瘤中呈高表达.产品图片Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-SPARC Polyclonal Antibody, Unconjugated（bs-1133R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-SPARC Polyclonal Antibody, Unconjugated（bs-1133R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingAntigen: bs-1133P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.