Gene Knockout Animal/Cell Line Models

Gene knockout models are cost-effective and can be delivered as early as 3 months （animal models） or 3 weeks （cell lines）.

Gene sequence has been revealed using whole genome sequence technology. However, function of a specific gene has far yet been elucidated. Many diseases have no cure medication and many unknown diseases have not been discovered. Gene knockout animal model is useful tool to confirm the functions of a specific gene, to discover the novel functions of a gene, to explore novel medication of a disease or to discover novel diseases.

We have developed cut-edge technology that can delete any gene in any tissue/organ or cell type. With many years of successful experience, we promise timely delivery of gene knockout mouse/rat models with deletion efficiency of more than 99.9%. We can also create gene-deficient cell lines that lack expression of gene of interest, such cell lines include embryonic stem cell （ESC）, induced pluripotent stem cell （iPSC）, fibroblast, adult stem cells, somatic cells and many other cell types. The gene knockout animals can be delivered as short as 3 months while gene knockout cell lines can be delivered as early as 3 weeks.

The CRISPR/Cas9 technology employs the RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats （CRISPR） adaptive immune system to cleave foreign genetic elements by simply specifying a 20-nt targeting sequence within its guide RNA （1, 2）. The Cas9-mediated genome editing technology uses nonhomologous end joining （NHEJ） or homology-directed repair （HDR） in mammalian cells, as well as generation of modified cell lines for downstream functional studies. The advantage of this novel technology over the other well developed such as TALENs （transcription activator-like effector nucleases） （3-5） or ZFNs （zinc-finger nucleases） （6） is that the CRISPR/Cas9 technology can delete multiple genes in a genome system, resulting in multiple gene knockout models （7）.

References

1. Garneau J.E. et al. （2010） The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67-71.

2. Horvath P & Barrangou R. （2010） CRISPR/Cas, the immune system of bacteria and archae. Science 327:167-170.

3. Hockemeyer D et al. （2011） Genetic engineering of human pluripotent cells using TALE nucleases. Nature Biotechnology 29（8）:731-734.

4. Tesson L. et al. （2011） Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology 29（8）:695-696.

5. Sander JD et al. （2011） Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 209（8）:697-698.

6. Watanabe M et al. （2013） Generation of interleukin-2 receptor gamma gene knockout pigs from somatic cell genetically modified by zinc finger nuclease-encoding mRNA. PLos One 8（10）:e76478.

7. Zhou J et al. （2013） One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering. Int J Biochem Cell Biol

原创作者：烟台基诺莱斯生物科技有限公司