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HIV-1 TAT Clade-C/重组HIV-1 TAT分化枝C
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HIV-1 TAT Clade-C/重组HIV-1 TAT分化枝C
产品编号：HIV-116
产品规格：2μg/10μg/100μg
Introduction Human immunodeficiency virus type-1 （HIV-1） regulatory Tat protein plays an essential role in viral replication ａnd infectivity. In addition, during acute infection, Tat is released extracellularly by infected cells ａnd is taken up by neighboring cells where it transactivates viral replication ａnd increases virus infectivity.
HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain （CTD） of RNA polymerase II （RNAPII）. Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes ａnd by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization. Description HIV-1 TAT Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 100 amino acids encoded by two exons ａnd having chain having a molecular mass of 21 kDa. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation HIV-1 TAT was lyophilized with no additives. Purity Greater than 90.0% as determined by HPLC analysis ａnd SDS-PAGE. Solubility It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 TAT should be stored at 4°C between 2⁻⁷ days ａnd for future use below -18°C.
For long-term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cyclesSpecificity Immunoreactive with all sera of HIV-1 infected individuals. Applications Recognized by anti-Tat （HIV-1） polyclonal antibody.
Reacts with anti-Tat antibodies from human, monkey, rabbit ａnd mouse serum. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：2μg/10μg/100μg
| Introduction | Human immunodeficiency virus type-1 （HIV-1） regulatory Tat protein plays an essential role in viral replication ａnd infectivity. In addition, during acute infection, Tat is released extracellularly by infected cells ａnd is taken up by neighboring cells where it transactivates viral replication ａnd increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain （CTD） of RNA polymerase II （RNAPII）. Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes ａnd by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization. |
| Description | HIV-1 TAT Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 100 amino acids encoded by two exons ａnd having chain having a molecular mass of 21 kDa. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | HIV-1 TAT was lyophilized with no additives. |
| Purity | Greater than 90.0% as determined by HPLC analysis ａnd SDS-PAGE. |
| Solubility | It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 TAT should be stored at 4°C between 2⁻⁷ days ａnd for future use below -18°C. For long-term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. |
| Please prevent freeze-thaw cyclesSpecificity | Immunoreactive with all sera of HIV-1 infected individuals. |
| Applications | Recognized by anti-Tat （HIV-1） polyclonal antibody. Reacts with anti-Tat antibodies from human, monkey, rabbit ａnd mouse serum. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
HIV-1,TAT,Clade-C,重组HIV-1,TAT分化枝C
