【文献标题】Anti-Inflammatory Activity of Water-Soluble Polysaccharide of Agaricus blazei Murill on Ovariectomized Osteopenic Rats
【作者】Peng Wang, Xiao-Tao Li, Lei Sun et.al
【作者单位】Harbin Medical University（哈尔滨医科大学）
【文献中引用产品】
大鼠白介素1β (IL-1β)ELISA试剂盒
大鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒
【关键词】无
【DOI】http://dx.doi.org/10.1155/2013/164817
【出版期刊】《Evidence-Based Complementary and Alternative Medicine》
【产品原文引用】
Estimation of Serum IL-1β and TNF-α Level. Serum samples were disintegrated in 5 volumes of ice-cold RIPA buffer. After incubation on ice for 30 minutes, samples were centrifuged twice at 20,000 Xg for 15 minutes at 4°C. The resulting supernatants were used for assay. The concentration of IL-1β and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.
【作者单位】哈尔滨医科大学第--附属医院（The First Affiliated Hospital of Harbin Medical University）
【文献中引用产品】
小鼠溴脱氧核苷尿嘧啶(BrdU)ELISA试剂盒
【DOI】https://doi.org/10.1038/labinvest.2015.106
【影响因子(IF)】5.51
【出版期刊】《LaboratoryInvestigation》
【产品原文引用】VSMC proliferation was analyzed via measuring DNA
synthesis with a colorimetric bromodeoxyuridine (BrdU)
ELISA kit according to the manufacturer’s instructions
(Shanghai JinMa Laboratory and Equipment Company,
Shanghai, China).
【文献标题】Tissue factor pathway inhibitor gene transfer prevents vascular smooth muscle cell proliferation by interfering with the MCP-3/CCR2 pathway
【作者】Yu Fu, Dandan Ma, Yue Liu,et.al
【作者单位】哈尔滨医科大学( Harbin Medical University)
【文献中引用产品】
小鼠溴脱氧核苷尿嘧啶(BrdU)ELISA试剂盒
小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒
【DOI】doi:10.1038/labinvest.2015.106
【影响因子(IF)】6.7
【出版期刊】《Laboratory Investigation》
【产品原文引用】
Cell Proliferation StudiesVSMC proliferation was analyzed via measuring DNA synthesis with a colorimetric bromodeoxyuridine (BrdU) ELISA kit according to the manufacturer’s instructions (Shanghai JinMa Laboratory and Equipment Company,Shanghai, China). Briefly, VSMCs were seeded in 24-well plates at a density of 5 × 104 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, after which the cell culture supernatants were collected and centrifuged at 12 000 g for 15 min at 4 °C; the extracted supernatants were immediately frozen at ? 80 °C until use. Absorbance values at 450 nm were determined using an ELISA reader (Bio-Rad, CA, USA).
【文献标题】Effects of Selenium-Enriched Agaricus blazei Murill on Liver Metabolic Dysfunction in Mice, a Comparison with Selenium-Deficient Agaricus blazei Murill and Sodium Selenite
Estimation of Liver IL-1β and TNF-α Level Liver samples were disintegrated in five volumes of ice-cold RIPA buffer. After incubation on ice for 30 min, samples were centrifuged twice at 20,000×g for 15 min at 4 °C. The resulting supernatants were used for assay. The hepatic concentration of interleukin-1β (IL-1β) and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.
在线咨询5分钟应答
