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89874 Mitochondria Isolation Kit for Cultured Cells

Name: 89874 Mitochondria Isolation Kit for Cultured Cells
Price: 3995 CNY

点击次数：254

价格：￥3995

更新日期：2026/9/8 5:10:31

生产地：中国大陆

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简单介绍：蛋白样品制备和蛋白质纯化，The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell sa

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相关标签：89874,Mitochondria,Isolation,Kit,for,Cultured,Cells

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描述

The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

Features of the Mitochondria Isolation Kit for Cultured Cells:

• Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
• Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
• Optimizable—guidelines provided for optimizing purity vs. yield with the reagent-based method
• Benchtop-easy—isolation performed in a microcentrifuge tube
• Compatible—isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis

Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity.

The product instructions describe guidelines for optimizing purity vs. yield parameters. The kit also offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondria recovery compared to the reagent-based method. Both methods use differential centrifugation to separate the mitochondrial ａnd cytosolic fractions with a benchtop microcentrifuge ａnd are completed in approximately 40 minutes (post-cell harvest). Once isolated, the mitochondria can be used in downstream applications such as apoptosis, signal transduction ａnd metabolic studies, as well as to facilitate mitochondrial proteomics efforts.