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Pierce™ Classic Magnetic IP/Co-IP Kit 88804

Name: Pierce™ Classic Magnetic IP/Co-IP Kit 88804
Price: 5292 CNY

点击次数：446

价格：￥5292

更新日期：2026/9/8 5:10:31

生产地：中国大陆

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简单介绍：The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using le

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相关标签：Pierce™,,Classic,Magnetic,IP/Co-IP,Kit,88804

详细内容

描述

The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective ａnd efficient immunoprecipitation (IP) ａnd co-immunoprecipitation (co-IP) of antigens ａnd protein-complexes using less than 10 µg of antibody ａnd a magnetic separator.

Features of the Classic Magnetic IP/Co-IP Kit:

• Compatible—magnetic beads based on Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit
• Fast—immunoprecipitate in as few as 30 minutes to reduce background ａnd improve the capture of transient protein complexes
• Clean—immobilize your antibody to prevent contamination in your eluate
• Resistant—no leaching of Protein A/G in the presence of detergents, low pH buffers or common mass spectrometry solvents
• Efficient—immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads
• Convenient—the comprehensive Co-IP kit contains the magnetic beads ａnd all essential buffers to complete immunoprecipitation experiments

This immunoprecipitation kit uses high-quality Thermo Scientific Pierce Protein A/G Magnetic Beads ａnd optimized buffers ａnd flexible protocol to accomplish high-yield IP or co-IP with manual or automated magnetic-separation tools. The optimized Lysis/Wash Buffer optimizes yield ａnd efficient binding of antibody-antigen ａnd co-IP interactions. The relatively gentle, low-pH Elution Buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the included Lane Marker Sample Buffer provides rapid, denaturing elution for direct SDS-PAGE analysis of IP products.

Includes:
Pierce Protein A/G Magnetic Beads, lysis/wash buffer, elution buffer, neutralization buffer ａnd sample loading buffer

Immunoprecipitation with magnetic particles is performed much like IP with beaded agarose, except that separations are performed using a magnet rather than by centrifugation. The specific antibody is first added to the sample to form an immune complex that is then bound to the magnetic beads. The complex is washed to remove non-bound material ａnd a low-pH elution buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the Lane Marker Sample Buffer is included for dissociation using denaturing conditions or for downstream sample prep for SDS-PAGE analysis. T

规格

Assay Type:	Affinity Purification
For Use With (Equipment):	KingFisher™ Flex, Magnetic Stand
Product Line:	Pierce™
Product Size:	40 reactions
Target Specificity:	Not Target-Specific
Technique:	Immunoprecipitation (IP)

内容及储存

Sufficient For: 40 IP reactions using 25 µL of beads
• Pierce Protein A/G Magnetic Beads, 1 mL
• Pierce IP Lysis/Wash Buffer, 2 x 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Elution Buffer, 5 mL
• Neutralization Buffer, 0.5 mL

Store at 4°C.

Pierce™,,Classic,Magnetic,IP/Co-IP,Kit,88804