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Pierce™ Co-IP Kit 26149

Name: Pierce™ Co-IP Kit 26149
Price: 4466 CNY

点击次数：473

价格：￥4466

更新日期：2026/9/8 5:10:31

生产地：中国大陆

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简单介绍：Pierce™ Co-IP Kit 26149

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相关标签：Pierce™,,Co-IP,Kit,26149

详细内容

描述

The Thermo Scientific Pierce Co-Immunoprecipitation Kit provides covalent antibody immobilization ａnd all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) ａnd co-immunoprecipitate any interacting proteins (prey proteins). Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy ａnd light chains that may co-migrate with relevant bands, masking important results. The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin. The kit includes optimized buffers for protein binding ａnd recovery, reagents to perform control experiments ａnd efficient spin columns ａnd collection tubes, which shorten the protocol ａnd minimize handling ａnd mixing.

Features of the Co-Immunoprecipitation Kit:

• Works for any purified antibody—perform co-IP experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A ａnd Protein G affinity binding
• No antibody interference—covalent attachment method ａnd non-reducing elution system yields pure co-IP products without contamination by the IP antibody
• Reusable antibody beads—covalent antibody immobilization allows prepared IP resin to be reused ａnd provides potential savings of costly antibody
• Complete kit—all reagents for antibody immobilization ａnd many IP experiments, including binding ａnd wash buffers, elution buffer ａnd convenient microcentrifuge spin columns to make resin manipulations easier ａnd more efficient.
• Control beads included—underivatized agarose beads provided for use as negative control for nonspecific binding
• Versatile—co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen ａnd protein interaction complex.

Co-immunoprecipitation (Co-IP) is a popular in-vitro method for discovering protein interactions. The Pierce Co-Immunoprecipitation Kit is configured to provide the essential tools to effectively perform Co-IP experiments in a manner that yields pure IP ａnd co-IP products free of troublesome IP antibody. This improvement on the traditional co-IP technique is achieved by replacing Protein A/G agarose beads with AminoLink Plus Resin to which pure IP antibodies can be directly ａnd permanently conjugated. Gentle (non-reducing, non-denaturing) elution buffer allows dissociation of the IP targets while retaining the immobilized antibody on the agarose beads. The result is a pure product that can be analyzed by Western blotting (or even sequenced) without interference from antibody.

规格

Assay Type:	Affinity Purification
For Use With (Equipment):	Microcentrifuge
Product Line:	Pierce™
Product Size:	50 reactions
Target Specificity:	Not Target-Specific
Technique:	Immunoprecipitation (IP)

内容及储存

Sufficient For: Preparing ａnd performing 50 co-IP reactions, each with 25 µL of resin
• AminoLink Plus Coupling Resin, 2 mL
• Coupling Buffer (20X), 25 mL
• Sodium Cyanoborohydride Solution (5M), 0.5 mL
• Quenching Buffer, 50 mL
• Wash Solution, 60 mL
• IP Lysis/Wash Buffer, 2 x 50 mL
• Modified Dulbecco＇s PBS (20X), 25 mL
• Conditioning Buffer (100X), 5 mL
• Elution Buffer, 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Pierce Spin Columns, Screw Cap, 50 columns
• Microcentrifuge Collection Tubes, 2 mL, 100 tubes
• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes
• Pierce Control Agarose Resin, 2 mL

Store at 4°C.

Pierce™,,Co-IP,Kit,26149