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87787 Pierce™ IP 裂解缓冲液 87788

Name: 87787 Pierce™ IP 裂解缓冲液 87788
Price: 1335 CNY

点击次数：836

价格：￥1335

更新日期：2026/9/8 5:10:31

生产地：中国大陆

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简单介绍：87787 Pierce™ IP 裂解缓冲液 87788

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描述

Thermo Scientific Pierce IP Lysis Buffer is optimized for cell lysate yield, purity ａnd compatibility with immunoprecipitation (IP ａnd Co-IP) as the downstream application for the cell lysate.

Features of IP Lysis Buffer:

• Optimized for compatibility with immunoprecipitation ａnd pull-down assays
• Compatible with protein assays, reporter assays ａnd immunoassay procedures
• Ready-made formula is effective for extracting cytoplasmic, membrane ａnd nuclear proteins
• Gentle formulation helps maintain protein complexes for co-immunoprecipitation
• Does not liberate genomic DNA which can cause high sample viscosity

IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pull-down ａnd immunoprecipitation assays.

Pierce IP Lysis Buffer is effective for lysing cultured mammalian cells from both plated cells ａnd cells pelleted from suspension cultures. Optimized for pull-down ａnd immunoprecipitation assays, this lysis buffer is also compatible with many other applications, including the Thermo Scientific Pierce BCA ａnd 660 nm Protein Assays, protein purification ａnd immunoassays (e.g., ELISA, Western blot).

Pierce IP Lysis Buffer is composed of 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 ａnd 5% glycerol. The buffer does not contain protease or phosphatase inhibitors; however, if desired, inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail or Phosphatase Inhibitor Cocktail can be added just before use to prevent proteolysis ａnd maintain phosphorylation of proteins.