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23227 Pierce™ BCA Protein Assay Kit 23225

Name: 23227 Pierce™ BCA Protein Assay Kit 23225
Price: 1814 CNY

点击次数：315

价格：￥1814

更新日期：2026/9/8 5:10:31

生产地：中国大陆

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简单介绍：23227 Pierce™ BCA Protein Assay Kit 23225

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相关标签：23227,Pierce™,,BCA,Protein,Assay,Kit,23225

详细内容

描述

Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Features of the BCA Protein Assay Kit include:
• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic ａnd non-ionic detergents
• Assay time—30-min incubation; much easier ａnd four times faster than classical Lowry methods
• Assay range—linear working range for BSA of 20 to 2000 µg/mL
• Sensitivity—detect down to 5 µg/mL with enhanced protocol

How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive ａnd selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble ａnd exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, ａnd tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

规格

Assay Range:	20-2,000µg/mL or 5-250µg/mL
Assay Type:	Protein Quantitation Assay
Detection Method:	Colorimetric (562 nm)
For Use With (Equipment):	Microplate Reader, Spectrophotometer
Product Line:	Pierce™
Product Size:	500 mL
Technique:	Absorbance, Solution-based Detection

内容及储存

Sufficient For: 250 tube assays or 2500 microplate assays
• BCA Reagent A, 500 mL
• BCA Reagent B, 25 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL

Store at room temperature

23227,Pierce™,,BCA,Protein,Assay,Kit,23225