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Maxima™ H Minus cDNA合成预混液，含dsDNase M1681

Name: Maxima™ H Minus cDNA合成预混液， 含dsDNase M1681
Price: 4066 CNY

点击次数：241

价格：￥4066

更新日期：2026/9/8 5:10:31

生产地：美洲

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简单介绍：Maxima™ H Minus cDNA合成预混液，含dsDNase M1681

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相关标签：Maxima™,,H,Minus,cDNA合成预混液,,含dsDNase,,M1681

详细内容

描述

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination ａnd cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete ｍaster mix that is convenient to use, reduces pipeting steps, ａnd is optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima H Minus cDNA Synthesis Master Mix is optimized for reproducible cDNA synthesis at elevated temperatures (50–65°C) within 30 minutes.

Features include:
• One-tube ｍaster mix for reduced pipetting steps ａnd enhanced consistency in RT-qPCR results
• Increased RT efficiency across a wide range of input RNA amounts ａnd gene targets
• High thermostability to allow RT reactions at 50 to 65°C temperature range
• Integrated step of genomic DNA removal from RNA samples

The ｍaster mix contains Maxima H Minus Reverse Transcriptase (RT) ａnd RiboLock RNase Inhibitor. Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, ａnd lack of RNase H activity. The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, ａnd C at temperatures up to 55°C. The ｍaster mix also contains reaction buffer, dNTPs, oligo (dT)18, ａnd random hexamer primers. A separate tube of No-RT control mix is provided for convenient RT negative control.

The included double-strand specific DNase (dsDNase) allows removal of genomic DNA from RNA samples in 2 minutes without affecting the quality or quantity of RNA. This dsDNase is also available separately (Cat. No. EN0771).

Additional information about reaction components
• The No RT Control mix contains all components in the Maxima H Minus cDNA Synthesis Master Mix except Maxima H Minus RT. The presence of a PCR product in the No RT Control reaction indicates that the reaction is contaminated with DNA. To further enhance genomic DNA elimination efficiency, template RNA incubation with the included dsDNase is strongly recommended.

规格

Reverse Transcriptase:	Maxima H Minus
Downstream Application:	Real-Time PCR
Reaction Format:	SuperMix or Master Mix
Sample Type (General):	RNA
Optimal Reaction Temperature:	50°C inculbation temperature for RT reactions
Product Line:	Maxima&trade
Product Size:	50 reactions
Shipping Condition:	Dry Ice

内容及储存

• 1 x 200 µL Maxima H Minus cDNA Synthesis Master Mix
• 1 x 200 µL Maxima H Minus cDNA Synthesis Master Mix No RT Control
• 1 x 50 µL dsDNase
• 1 x 100 µL 10X dsDNase buffer
• 1 x 1.25 mL nuclease-free water

Store at -5 to -30°C.

Maxima™,,H,Minus,cDNA合成预混液,,含dsDNase,,M1681