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美国ABI Proteinase K Solution AM2548

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价格：￥电议

更新日期：2026/9/8 5:10:31

生产地：美洲

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简单介绍：Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active

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详细内容

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range (optimal activity between 6.5 ａnd 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA ａnd RNA; preparation of tissue sections for in situ hybridization.

Performance ａnd quality testing
Endodeoxyribonuclease ａnd exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.