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美国ABI Proteinase K蛋白酶K25530015

点击次数：550

价格：￥电议

更新日期：2026/9/8 5:10:31

生产地：美洲

产品型号：25530015

简单介绍：Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active

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详细内容

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and

9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA ａnd RNA; preparation of tissue sections for in situ hybridization.

Performance ａnd quality testing
Endodeoxyribonuclease ａnd exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.