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美国ABI SilverQuest™ Silver Staining KitLC6070
点击次数：915
价格：￥电议
更新日期：2026/9/8 5:10:23
生 产 地：美洲
产品型号：LC6070
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相关标签：SilverQuest™,,Silver,Staining,Kit
详细内容
The SilverQuest™ Silver Staining Kit provides a rapid ａnd easy method to silver stain proteins ａnd DNA in polyacrylamide gels. Silver staining allows detection of most proteins since it is 30-fold more sensitive than staining with colloidal Coomassie™ G-250. The SilverQuest™ Silver Staining Kit is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.
• High sensitivity – Protein detection at sub-nanogram level
• Compatible – Staining compatible with mass spectrometry analysis
• Rapid – Protein bands can be visualized within an hour
• Easy to use – Color-coded solutions for easy staining protocol
The SilverQuest™ Silver Staining Kit is based on the chemical reduction of silver ions to metallic silver on a protein band. The protein bands are visualized as spots where reduction begins. For more details on the mechanism of silver staining, refer to Rabilloud et al, 1994.
Ideal for Rapid Preparation of Samples for Mass Spectrometry
The mass spectrometry problems associated with traditional silver stains are mitigated with the SilverQuest™ Silver Staining Kit because the reagents used do not modify protein side chains ａnd do not interfere with trypsin digestion. Typically, in less than 90 minutes, 0.3 ng of protein can be detected (Figure 1). For added speed, an optimized microwave procedure can typically be completed in 30 minutes without compromising sensitivity.
Mass Spectrometry-Compatible Reagents
The SilverQuest™ Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest™ reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so as to leave lysine ａnd arginine side-chains unmodified. Additionally, the SilverQuest™ destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion ａnd gel extraction, ａnd yield better coverage during mass spectrometry
• High sensitivity – Protein detection at sub-nanogram level
• Compatible – Staining compatible with mass spectrometry analysis
• Rapid – Protein bands can be visualized within an hour
• Easy to use – Color-coded solutions for easy staining protocol
The SilverQuest™ Silver Staining Kit is based on the chemical reduction of silver ions to metallic silver on a protein band. The protein bands are visualized as spots where reduction begins. For more details on the mechanism of silver staining, refer to Rabilloud et al, 1994.
Ideal for Rapid Preparation of Samples for Mass Spectrometry
The mass spectrometry problems associated with traditional silver stains are mitigated with the SilverQuest™ Silver Staining Kit because the reagents used do not modify protein side chains ａnd do not interfere with trypsin digestion. Typically, in less than 90 minutes, 0.3 ng of protein can be detected (Figure 1). For added speed, an optimized microwave procedure can typically be completed in 30 minutes without compromising sensitivity.
Mass Spectrometry-Compatible Reagents
The SilverQuest™ Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest™ reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so as to leave lysine ａnd arginine side-chains unmodified. Additionally, the SilverQuest™ destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion ａnd gel extraction, ａnd yield better coverage during mass spectrometry
规格
| Target Molecule: | Protein |
|---|---|
| Label or Dye: | SilverQuest™ Silver Stain |
| Detection Method: | Colorimetric |
| Detection Location: | In-Gel Detection |
| Product Size: | 1 kit |
内容及储存
The SilverQuest™ Silver Staining Kit is supplied with sufficient sensitizer, stainer, developer, stop solution, ａnd destaining solutions for 25 mini-gels. Store the kit at room temperature. Guaranteed stable for 6 months when properly stored.
SilverQuest™,,Silver,Staining,Kit
