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美国ABI TA Cloning® Kit, with pCR™2.1 Vector,

点击次数：770

价格：￥电议

更新日期：2026/9/8 5:10:23

生产地：美洲

产品型号：K202020

简单介绍：The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. The TA Clon

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相关标签：TA,Cloning®,,Kit,,with,pCR™,2.1,Vector,,without,competent,cells

详细内容

The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. The TA Cloning® Kit uses the pCR™2.1 cloning vector ａnd ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

Features of the TA Cloning® Kit with pCR™2.1 vector:
• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening ａnd >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites

The pCR™2.1 vector provides:
• 3＇-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription ａnd sequencing
• A versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward ａnd reverse primer sites for sequencing

How TA Cloning® Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3＇ ends of PCR products. The linearized vector supplied in this kit has single 3＇ deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Kit Configurations
The TA Cloning® Kit is offered in a variety of configurations: without competent cells (K2020-20 ａnd K2020-40), with One Shot® INVF＇ Chemically Competent E. coli (K2000-01 ａnd K2000-40), with One Shot® TOP10F＇ Chemically Competent E. coli (K2030-01 ａnd K2030-40), ａnd with One Shot® TOP10 Chemically Competent E. coli (K2040-01 ａnd K2040-40) in 20- ａnd 40-reaction kit sizes.

规格

Vector Type:	TA Cloning Vectors
Cloning Method:	TA Cloning®
Bacterial or Yeast Strain:	Not Included
Product Size:	20 reactions

内容及储存

TA Cloning® kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, ａnd controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.