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美国ABI Zero Blunt® TOPO® PCR Cloning Kit, 450245

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价格：￥电议

更新日期：2026/9/8 5:10:23

生产地：中国大陆

产品型号：450245

简单介绍：Zero Blunt® TOPO® PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of blunt-ended PCR prod

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相关标签：Zero,Blunt®,,TOPO®,,PCR,Cloning,Kit,,without,competent,cells

详细内容

ero Blunt® TOPO® PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has the Zero Blunt® TOPO® vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. Zero® Blunt® TOPO® kits are available with a variety of competent cells, or without competent cells, depending on your needs ａnd budget. Features of the Zero® Blunt® TOPO® PCR Cloning Kits:

Fast ａnd easy—go from PCR to clones in just 3 steps ａnd in as little as 5 minutes hands-on time
Efficient—obtain up to 95% clones with correct insert
Proven—reliable performance for over a decade with over 4,000 citations
Simple —no ligase, post-PCR procedures, or PCR primers containing specific sequences are required.

Zero Blunt® TOPO® Cloning Kits—overview

Vector: pCR®Blunt II-TOPO® vector—contains the ccdB gene, allowing for direct selection of recombinants

Cloning method: TOPO® cloning—Topoisomerase I–based, 5-minute ligation of blunt-ended PCR products amplified with proofreading thermostable polymerases

Competent cells: Various options—choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells, or use your own.

pCR®Blunt II-TOPO® vector—designed for proofreading polymerases
The pCR®Blunt II-TOPO® vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum® Pfx DNA Polymerase. The pCR®Blunt II-TOPO® vector contains:

• EcoRI sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin ａnd Zeocin™ resistance genes for your choice of selection in E. coli
• SP6 promoter/primer site for in vitro RNA transcription ａnd sequencing
• M13 forward ａnd reverse primer sites for sequencing or PCR screening

pCR®Blunt II-TOPO® clone selection
pCR®-Blunt II-TOPO® allows for direct selection of recombinants via disruption of the lethal E. coli gene, ccdB. The vector contains the ccdB gene fused to the C-terminus of the LacZα fragment. Ligation of a blunt-end PCR product disrupts expression of the LacZα-ccdB gene fusion, permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.

Simplified TOPO®-based cloning
Using TOPO® cloning technology, there is no need for PCR primers containing specific sequences, post-PCR procedures, vector preparation, or other time-intensive DNA manipulation steps. Just add your PCR reaction straight to the provided topoisomerase-charged vector, incubate 5 minutes, ａnd transform E. coli competent cells.

规格

Number of Reactions:	20 Reactions
Vector Type:	Blunt DNA Cloning Vectors, pCR, TOPO Cloning Vectors
Cloning Method:	Blunt TOPO®
Bacterial or Yeast Strain:	Not Included
Antibiotic Resistance (Bacterial):	Kanamycin (KanR), Zeocin™ (ZeoR)
PCR Enzyme:	Proof-Reading Polymerase
Product Size:	25 reactions
Directionality:	No
Replication Origin:	pUC Origin
Shipping Condition:	Dry Ice

内容及储存

Box 1:
• Linearized ａnd topoisomerase I-activated pCR™Blunt II-TOPO® vector
• Salt solution
• dNTPs
• Control template
• M13 forward ａnd reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

450245

Zero Blunt® TOPO® PCR 平端克隆试剂盒（pCR® Blunt II TOPO® vector）

2918

Zero,Blunt®,,TOPO®,,PCR,Cloning,Kit,,without,competent,cells