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美国ABITaqMan® Genotyping Master Mix4371353 4371355 43
点击次数：1038
价格：￥电议
更新日期：2026/9/8 5:10:23
生 产 地：美洲
产品型号：4381656
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相关标签：TaqMan®,,Genotyping,Master,Mix
详细内容
The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.
• Distinct clusters ａnd high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- ａnd post-PCR stability for high throughput setup ａnd analysis
May be used for a variety of genotyping applications, including:
• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications
Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.
Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling ａnd inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays
Validated with TaqMan® Genotyping Assays ａnd Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, ａnd Custom TaqMan® SNP Genotyping Assays. In addition, the ｍaster mix is validated with the Applied Biosystems® thermal cyclers ａnd real-time PCR systems.
High-Throughput Setup ａnd Analysis
The combination of several low-cost thermal cyclers for PCR ａnd a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup ａnd analysis with excellent room temperature stability both before ａnd after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.
Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters ａnd more accurate allele-calls compared to other mixes, even for challenging targets.
Setting the Industry Standard for Quality
Extensive analytical ａnd functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay ａnd one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for ＇Certificate of Analysis＇).
Number of Genotyping Reactions at a Given Reaction Size:
4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000
• Distinct clusters ａnd high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- ａnd post-PCR stability for high throughput setup ａnd analysis
May be used for a variety of genotyping applications, including:
• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications
Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.
Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling ａnd inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays
Validated with TaqMan® Genotyping Assays ａnd Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, ａnd Custom TaqMan® SNP Genotyping Assays. In addition, the ｍaster mix is validated with the Applied Biosystems® thermal cyclers ａnd real-time PCR systems.
High-Throughput Setup ａnd Analysis
The combination of several low-cost thermal cyclers for PCR ａnd a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup ａnd analysis with excellent room temperature stability both before ａnd after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.
Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters ａnd more accurate allele-calls compared to other mixes, even for challenging targets.
Setting the Industry Standard for Quality
Extensive analytical ａnd functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay ａnd one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for ＇Certificate of Analysis＇).
Number of Genotyping Reactions at a Given Reaction Size:
4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000
规格
| Platform: | 7300 System, 7000 System, 7900HT System, StepOnePlus™, Standard Mode, StepOne™, Standard Mode, 7500 System |
|---|---|
| Technique: | CNV (Copy Number Variation), SNP Genotyping (Real-Time PCR-Based) |
| PCR Method: | qPCR |
| Polymerase: | AmpliTaq Gold® DNA Polymerase |
| Product Size: | 1 x 1 mL |
| Reaction Speed: | Standard |
| Detection Method: | Primer-Probe Detection |
| Genotyping Target: | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Sample Type (General): | DNA |
| Sample Type (Specific): | DNA (Genomic) |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Primer-Probe Compatibility: | TaqMan® |
内容及储存
Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan® probes ａnd contains AmpliTaq Gold® DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 ａnd optimized mix components. This pack contains one 1 mL tube, sufficient for 40 reactions at the 50 µL total volume.
Storage: 2-8°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product ａnd CofA).
Storage: 2-8°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product ａnd CofA).
| 4371353 | TaqMan® Genotyping Master Mix, Mini-Pack (1mL) | 100 rxn | 1119 |
| 4371355 | TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL) | 1000 rxn | 8680 |
| 4381656 | TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL) | 2000 rxn | 15760 |
TaqMan®,,Genotyping,Master,Mix
