联系我们

联系人：赵阳

点击查看联系方式

供应分类

产品展示

AAT FCB [Fluorescein di-beta-D-cellobioside]

点击次数：377

价格：￥电议

更新日期：2026/9/8 5:11:04

生产地：中国大陆

产品型号：14025

简单介绍：14025 AAT FCB [Fluorescein di-beta-D-cellobioside]

商家名片

赵*

联系我时，请说明是阿仪网看到的，谢谢!

电话: 01*******053
手机: 18******805
微信:
交换名片后展示联系方式 → →

相关标签：14025,,AAT,FCB,[Fluorescein,di-beta-D-cellobioside]

详细内容

联系人：赵阳

咨询电话：

This non-fluorescent fluorescein substrate generates the bright fluorescein product that has Ex/Em = 492/514 nm, ａnd can be easily detected with a FITC filter set. In general, fluorescein substrates are much more sensitive than coumarin or nitrophenol-based substrates. This fluorescein substrate is used for monitoring cellulase activities. Cellulases are a family of enzymes that include β-glucosidases, endoglucanases ａnd exoglucanases. These enzymes cleave the β-1,4-D-glycosidic bonds that link the glucose units comprising cellulose. In addition to being produced by plants, cellulase activity is found in many fungi ａnd bacteria, including some plant pathogens. Most animal cells are not known to produce cellulase, in which the cellulolytic activity is often carried out by symbionts. The study of cellulase activity has many applications in plant molecular biology, agriculture, ａnd manufacturing. Cellulase is becoming important in the development of alternative fuel sources, as glucose obtained from cellulose hydrolysis is easily fermented into ethanol. Activity of most cellulases can be conveniently monitored using this sensitive fluorescein cellobioside. Upon cleavage, the fluorescent compound, fluorescein, is released ａnd activity measurements are easily obtained in a microtiter plate based assay format.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FCB [Fluorescein di-beta-D-cellobioside] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	101.95 µL	509.752 µL	1.02 mL	5.098 mL	10.195 mL
5 mM	20.39 µL	101.95 µL	203.901 µL	1.02 mL	2.039 mL
10 mM	10.195 µL	50.975 µL	101.95 µL	509.752 µL	1.02 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
<ｓelect id="calculator_stock_calc_mass_unit" style="font-family: "Open sans", sans-serif; font-size: 13px; padding: 3px; margin: 0px; color: rgb(34, 34, 34); max-width: 73px; border-color: rgb(207, 207, 207);">	/	<ｓelect disabled="" style="font-family: "Open sans", sans-serif; font-size: 13px; padding: 3px; margin: 0px; color: rgb(34, 34, 34); max-width: 73px; border-color: rgb(207, 207, 207);">	=	<ｓelect id="calculator_stock_calc_volume_unit" style="font-family: "Open sans", sans-serif; font-size: 13px; padding: 3px; margin: 0px; color: rgb(34, 34, 34); max-width: 73px; border-color: rgb(207, 207, 207);">	x	<ｓelect id="calculator_stock_calc_concentration_unit" style="font-family: "Open sans", sans-serif; font-size: 13px; padding: 3px; margin: 0px; color: rgb(34, 34, 34); max-width: 73px; border-color: rgb(207, 207, 207);">	=	<ｓelect id="calculator_stock_calc_moles_unit" disabled="" style="font-family: "Open sans", sans-serif; font-size: 13px; padding: 3px; margin: 0px; color: rgb(34, 34, 34); max-width: 73px; border-color: rgb(207, 207, 207);">

Spectrum

Open in: Advanced Spectrum Viewer

Cursor Location

Wavelength (nm)	330.94
Relative Intensity (%)	29.81

Images

Figure 1. Principle of InVitroFlow comprising 7 steps. (1) Mutant library generation using a linear DNA template (approx. 6 h), (2) entrapment of mutant cellulase library in (w/o) single emulsions within 0.5 h, (3) cell-free expression of mutant library ａnd generation of (w/o/w) emulsions within 4 h, (4) sorting of active variants within (w/o/w) emulsions using flow cytometer within 2 h, ａnd (5) DNA recovery from (w/o/w) emulsions ａnd PCR gene amplification in 3.5 h. A whole round of InVitroFlow (diversity generation, screening by flow cytometry, amplification) can be completed within 16 h. (6) Cloning ａnd transformation into expression host (2 days), ａnd (7) screening of up to 2,000 beneficial clones in MTP format ａnd characterization of a few variants (7–12 days). Source: In vitro flow cytometry-based screening platform for cellulase engineering by Körfer et al., Scientific Reports, May 2016.

Figure 2. Chemical structure for FCB [Fluorescein di-beta-D-cellobioside]

References

View all 26 references: Citation Explorer

In vitro flow cytometry-based screening platform for cellulase engineering
Authors: Körfer, Georgette ａnd Pitzler, Christian ａnd Vojcic, Ljubica ａnd Martinez, Ronny ａnd Schwaneberg, Ulrich
Journal: Scientific reports (2016)

Interaction of cellulase with cationic surfactants: using surfactant membrane selective electrodes ａnd fluorescence spectroscopy
Authors: Rastegari AA, Bordbar AK, Taheri-Kafrani A.
Journal: Colloids Surf B Biointerfaces (2009): 132

Immobilization of cellulose fibrils on solid substrates for cellulase-binding studies through quantitative fluorescence microscopy
Authors: Moran-Mirabal JM, Santhanam N, Corgie SC, Craighead HG, Walker LP.
Journal: Biotechnol Bioeng (2008): 1129

A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside
Authors: Coleman DJ, Studler MJ, Naleway JJ.
Journal: Anal Biochem (2007): 146

Analysis of cellulase synthesis mechanism in Trichoderma reesei using red fluorescent protein
Authors: Liu G, Zhang Y, Li Y, Yu SW, Xing M.
Journal: Wei Sheng Wu Xue Bao (2007): 69

Cellulase digestibility of pretreated biomass is limited by cellulose accessibility
Authors: Jeoh T, Ishizawa CI, Davis MF, Himmel ME, Adney WS, Johnson DK.
Journal: Biotechnol Bioeng (2007): 112

The linker region plays a key role in the adaptation to cold of the cellulase from an Antarctic bacterium
Authors: Sonan GK, Receveur-Brechot V, Duez C, Aghajari N, Czjzek M, Haser R, Gerday C.
Journal: Biochem J (2007): 293

Use of an enhanced green fluorescence protein linked to a single chain fragment variable antibody to localize Bursaphelenchus xylophilus cellulase
Authors: Zhang Q, Bai G, Cheng J, Yu Y, Tian W, Yang W.
Journal: Biosci Biotechnol Biochem (2007): 1514

Interaction of cellulase with sodium dodecyl sulfate at critical micelle concentration level
Authors: Xiang J, Fan JB, Chen N, Chen J, Liang Y.
Journal: Colloids Surf B Biointerfaces (2006): 175

Pathogenic cellulase assay of pine wilt disease ａnd immunological localization
Authors: Zhang Q, Bai G, Yang W, Li H, Xiong H.
Journal: Biosci Biotechnol Biochem (2006): 2727