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技术文章
Desmin/DES/FITC 标记抗体
点击次数：861 发布时间：2012/10/4 19:06:54
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Anti-Desmin/DES/FITC 标记抗体说明书
Catalog Number : RS-1026R-FITC
Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA ａnd 0.1% Sodium azide. ）
Background:filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other ａnd to the plasma membrane from the periphery of the Z line structures. Defects in Desmin are the cause of desmin related cardio skeletal myopathy （CSM） also known as desmin related myopathy （DRM）. CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, ａnd by intracytoplasmic accumulation of desmin reactive deposits in cardiac ａnd skeletal muscle cells. A desmin related myopathy can have a distal onset, it is then known as hereditary distal myopathy （HDM）. Defects in Desmin are also the cause of dilated cardiomyopathy type 1I （CMD1I）. CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation ａnd impaired systolic function. Antidesmin antibodies are useful in identification of tumours of myogenic origin
.
Specificity : Anti- desmin is a rabbit monoclonal antibody, FITC conjugated
· specific for desmin of rat
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt：52kDa
Anti-Desmin/DES/FITC 标记抗体说明书
Application : · FCM: 1:20-100
· Immunohistochemistry : 1:20-1:200
· Immunocytochemistry: 1:20-1:200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
· Optimal working dilutions must be determined by the end user.
· protect from light.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-Desmin/DES/FITC 标记抗体说明书
原创作者：上海广锐生物科技有限公司
