重组新冠病毒 B.1.1.529 (Omicron) Spike S1 蛋白 (His标签)

重组新冠病毒 B.1.1.529 (Omicron) Spike S1 蛋白 (His标签)（产品说明）

货号：40591-V08H41

蛋白名称：SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations A67V, HV69⁻⁷0 deletion, T95I, G142D, VYY143-145 deletion, N211 deletion, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529) which emerged in the South Africa.
表达宿主：HEK293 Cells
蛋白纯度：> 95% as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 B.1.1.529 (Omicron) S1 Protein (His Tag)(Cat:40591-V08H41), the EC50 of is 45-140 ng/mL.
蛋白内毒素：< 1.0 EU per µg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 Spike S1 consists of 678 amino acids and predicts a molecular mass of 76.48 kDa. As a result of glycosylation, it migrates as an approximately 99.56 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Arg685
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.