重组新冠病毒 Spike S1+S2 ECD(mutants)-His 蛋白

SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein: 产品信息

货号：40589-V08H4
纯度：> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
内毒素：< 1.0 EU per µg protein as determined by the LAL method.
生物活性：Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4) is 10-50 ng/mL.
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主：HEK293 Cells
种属：2019-nCoV
预测 N 端：Val 16
分子量：The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P), His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
缓冲液：Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶：A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.