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FGF-10重组蛋白
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更新日期：2026/8/7 18:57:27
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详细内容
| 品牌 | 自营品牌 | 货号 | IMF-F10 |
|---|---|---|---|
| 规格 | 10ug 20ug 50ug 100ug | 供货周期 | 现货 |
FGF-10重组蛋白
FGF-10重组蛋白说明书
▍Backgound
Keratinocye Growth Factor-2 （KGF-2）, also known as FGF-10, was originally identified from rat embryos by homology-based polymerase chain reaction. Human ａnd mouse KGF-2 were subsequently cloned. The human KGF-2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF-2 shares approximately 92% ａnd 95% amino acid sequence identity with mouse ａnd rat KGF-2, respectively. Among the FGF family members, KGF-2 is most closely related to FGF⁻⁷. The expression of KGF-2 transcripts has been shown to be most abundant in the embryo ａnd adult lung. Recombinant KGF-2 preparations have been shown to be mitogenic for epithelial ａnd epidermal cells but not fibroblasts. Based on its in vitro biological activities ａnd in vivo expression pattern, KGF-2 has been proposed to play unique roles in the brain, in lung development, wound healing ａnd limb bud formation.
▍Source
Recombinant Human FGF10/KGF 2 is expressed from Escherichia coli. It contains AA Gln 38 - Ser 208. Predicted molecular mass 22.1 kDa. This protein carries a polyhistidine tag at the N-terminus.
▍Endotoxin
Less than 1.0 EU per µg by the LAL method.
▍AA Sequence
QALGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
▍Purity
Purity >95% as determined by SDS-PAGE.
▍Formulation
Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
▍Reconstitution
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots ａnd stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
▍Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to ⁻⁷0°C for 12 months in lyophilized state;
⁻⁷0°C for 3 months under sterile conditions after reconstitution.
▍SDS-PAGE
Human FGF-10, His Tag on SDS-PAGE under reducing （R） condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
