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做好ELISA实验需要注意哪些细节?

点击次数:440 发布时间:2020/6/29 17:37:12
做好ELISA实验需要注意哪些细节?

想要正确的操作ELISA实验,操作当中的每一个步骤都非常重要,不可忽略任何一个操作细节。下面是具体步骤:

1.严格按照规定的时间和温度进行温育以保证准确结果。所有试剂都必须在使用前达到室温20-25℃。使用后立即冷藏保存试剂。

2. 洗板不正确可以导致不准确的结果。在加入底物前确保尽量吸干孔内液体。温育过程中不要让微孔干燥掉。

3. 消除板底残留的液体和手指印,否则影响OD值。

4. 底物显色液应呈无色或很浅的颜色,已经变蓝的底物液不能使用。

5. 避免试剂和标本的交叉污染以免造成错误结果。

6. 在储存和温育时避免强光直接照射。

7. 平衡至室温后再打开密封袋以防水滴凝聚在冷板条上。

8. 任何反应试剂不能接触漂白溶剂或漂白溶剂所散发的强烈气体。任何漂白成分都会破坏试剂盒中反应试剂的生物活性。

9. 不能使用过期产品。

10. 如果可能传播疾病,所有的样品都应管理好,按照规定的程序处理样品和检测装置。

 

11.试剂盒从冷藏环境中取出应在室温平衡后方可使用。

20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份20×洗涤缓冲液加19份蒸馏水。

 

Shanghai Zhen Ke Biological Technology Co., Ltd. is a high-tech biotechnology company specialized in product development, marketing and technical services. By biological relying on the advantage of technology research and development personnel at home and abroad, by the United States, a senior scientist and several domestic research for many years engaged in the biological field of doctor, master biology researchers founded in university, such as in the field of scientific research and medical testing enjoys a high reputation, since the company since its establishment, the company's sales to maintain high growth, enterprise scale, research and development products include elisa kit, animal blood product, antibodies, standard, test card, biochemical reagents such as experiment material, such as more than 100000 kinds, the company long-term committed to the development of elisa kit, biological reagents, We provide a series of experimental testing services for customers, such as radioimmunoassay kits, ELISA kits, liquid chromatography services.

 

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