Description
Overview	Novagen＇s T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7. This system is easy to use ａnd has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), ａnd peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage). Phage assembly takes place in the E. colicytoplasm ａnd mature phage are released by cell lysis. Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly. T7 has additional properties that make it an attractive display vector. It is very easy to grow ａnd replicates more rapidly than either bacteriophage l or filamentous phage. Plaques form within 3h at 37°C ａnd cultures lyse 1-2 h after infection, decreasing the time needed to perform the multiple rounds of growth usually required for selection. The T7 phage particle is extremely robust, ａnd is stable to harsh conditions that inactivate other phage. This stability expands the variety of agents that can be used in biopanning selection procedures, which require that the phage remain infective. T7 is an excellent general cloning vector. Purified DNA is easy to obtain in large amounts, a high-efficiency in vitro packaging system has been demonstrated (2), ａnd the DNA is completely sequenced (39,937 bp), so restriction or DNA sequence analysis of clones is quite straightforward. The T7Select Phage Display System uses the T7 capsid protein to display peptides or proteins on the surface of the phage. The capsid protein is normally made in two forms, 10A (344 aa) ａnd 10B (397 aa). 10B is produced by a translational frameshift at amino acid 341 of 10A, ａnd normally makes up about 10% of the capsid protein. However, functional capsids can be composed entirely of either 10A or 10B, or of various ratios of the proteins. This finding provided the initial suggestion that the T7 capsid shell could accommodate variation, ａnd that the region of the capsid protein unique to 10B might be on the surface of the phage ａnd could be used for phage display. There are 3 basic types of T7Select phage display vectors: the T7Select415 vector for high-copy number display of peptides, the T7Select10 vector for mid-copy number display of peptides or larger proteins, ａnd the T7Select1 vectors for low-copy number display of peptides or larger proteins. In all of the vectors, coding sequences for the peptides or proteins to be displayed are cloned within a series of multiple cloning sites following aa 348 of the 10B protein. The natural translational frameshift site within the capsid gene has been removed, so only a single form of capsid protein is made from these vectors. The T7Select System is sold for research use only. Any commercial use of this Product, including drug discovery or development of commercial products, requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Overview	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC ａnd 2009/41/EC of the European Parliament ａnd of the Council ａnd their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you ａnd the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number	70550
Brand Family	Novagen®

Product Information
•	5 µg	T7Select EcoR I/Hind III Vector Arms
•	0.2 pmol	T7Select Control Insert
•	6	T7 Packaging Extracts
•	1 µg	T7Select Packaging Control DNA
•	0.2 ml	BLT5403, BLT5615, ａnd BL21 glycerol stock
•	500 pmol	T7SelectUP primer
•	500 pmol	T7SelectDOWN primer

Storage ａnd Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ ⁻⁷0°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

merck,merck,millipore,,merck授权代理,merck经销商,merck一级代理

采购记录

优质供应

我要推广

优质信息推广广告

友情链接
热词推荐

已获得阿仪网信誉等级评定

产品展示

merck millipore T7Select 10-3 Cloning Kit 今日报道merck 70550

点击次数：440

联系我们

联系我们

Eaivelly

实验仪器

ELISA试剂盒

其他检测试剂盒

抗体

实验耗材

其他实验耗材

生物化学试剂

动物血清制品

二代测序

自主品牌

产品展示

merck millipore T7Select 10-3 Cloning Kit 今日报道

点击次数：440

详细内容

采购记录

优质供应

相关产品

相关文章

我要推广

已获得阿仪网信誉 等级评定

产品展示

merck millipore T7Select 10-3 Cloning Kit 今日报道merck 70550

点击次数：440

联系我们

联系我们

产品展示

merck millipore T7Select 10-3 Cloning Kit 今日报道

点击次数：440

详细内容

采购记录

优质供应

相关产品

相关文章

我要推广

已获得阿仪网信誉等级评定