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小鼠皮下结缔组织细胞株/菌种Wnt-3A（ATCC ® CRL-2647 ™）

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价格：￥电议

更新日期：2026/9/8 3:56:46

生产地：美洲

产品型号：Wnt-3A

简单介绍：Wnt-3A基因编码具有多种信号传导效应的分泌型糖蛋白。Wnt基因控制胚胎发育期间的许多图案和生长事件。

细胞分泌生物活性Wnt-3A蛋白。它们目前是生产Wnt-3A条件培养基的最佳来源。
由于条件培养基除了Wnt-3A蛋白质以外还包含其它因素，所以有必要通过控制来自亲本细胞系（ATCC CRL-2648）的条件培养基来控制涉及Wnt-3A条件培养基的任何实验。

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相关标签：小鼠皮下结缔组织细胞株/菌种Wnt-3A,Wnt-3A细株,ATCC细胞株,

详细内容

小鼠皮下结缔组织细胞株Wnt-3A
一、产品描述
OrganismMus musculus, mouseTissuesubcutaneous connective tissue; areolar ａnd adiposeProduct FormatfrozenMorphologyfibroblastCulture PropertiesadherentBiosafety Level1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age100 daysGendermaleStrainC3H/AnApplications

This cell line is presently the best source for production of Wnt-3A conditioned medium.

Storage Conditionsliquid nitrogen vapor phase

二、产品特点

Derivation	L-M(TK-) cells (ATCC CCL-1.3) were transfected with a Wnt-3A expression vector ａnd stable clones were selected in medium containing G418.
Clinical Data	male
Genes Expressed	Wnt-3A
Cellular Products	Wnt-3A protein
Comments	The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning ａnd growth events during embryonic development. The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium. Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line (ATCC CRL-2648).

三、培养方法及注意事项

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.4 mg/ml G-418; fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove ａnd discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by pipetting gently.
Add appropriate aliquots of cell suspension to new culture vessels.
Incubate culture vessels at 37°C.

Protocol for Wnt-3A Conditioned Medium:

Split the cells 1:10 in 10 mL culture medium (without G418 if conditioned medium is to be used with a cell line sensitive to G418) in 10 cm tissue culture dishes or T⁻⁷5 flasks ａnd let the cells grow for 4 days (approximately to confluency).
Take off the medium ａnd sterile filter. This is the first batch of medium.
Add 10 mL fresh culture medium ａnd culture for another 3 days.
Take off the medium ａnd sterile filter. This is the second batch of medium. Discard the cells, because they will be overgrown.
Mix the first batch ａnd second batch of medium 1:1. This is the Wnt-3A conditioned medium. It is stable at 4°C ａnd can be frozen.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended.

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation ａnd subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

小鼠皮下结缔组织细胞株/菌种Wnt-3A,Wnt-3A细株,ATCC细胞株,

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