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江苏荧光素酶(萤火虫)使用方法
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更新日期：2025/12/2 1:07:04
生 产 地：中国大陆
产品型号：ZH
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相关标签：试剂保存方法
详细内容
荧光素酶(萤火虫)
中文名称：荧光素酶(萤火虫)
其他中文名称： 荧光素酶；重组型酵素
英文名称： Luciferase firefly
其他英文名称： Luciferase from Photinus pyralis (firefly)
产品规格： BR，98%
包装： 1毫克
CAS号：61970-00-1
级别：BR
product line：BioUltra
荧光素酶(萤火虫)Assay：≥98% (SDS-PAGE)
Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein：10-35% E1%/280
Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase ａnd suitably sensitive liquid scintillation counters or luminometers)
Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP ａnd 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase ａnd nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein ａnd less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP ａnd 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性状：粉末。EC：1.13.12.7
用途：生化研究。
荧光素酶(萤火虫)保存：-20℃。保质期1年
中文名称：荧光素酶(萤火虫)
其他中文名称： 荧光素酶；重组型酵素
英文名称： Luciferase firefly
其他英文名称： Luciferase from Photinus pyralis (firefly)
产品规格： BR，98%
包装： 1毫克
CAS号：61970-00-1
级别：BR
product line：BioUltra
荧光素酶(萤火虫)Assay：≥98% (SDS-PAGE)
Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein：10-35% E1%/280
Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase ａnd suitably sensitive liquid scintillation counters or luminometers)
Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP ａnd 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase ａnd nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein ａnd less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP ａnd 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性状：粉末。EC：1.13.12.7
用途：生化研究。
荧光素酶(萤火虫)保存：-20℃。保质期1年
试剂保存方法
