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Annexin V-FITC 细胞凋亡检测试剂盒
点击次数：549
价格：￥电议
更新日期：2026/3/19 20:10:36
生 产 地：中国大陆
产品型号：556547
详细内容
商品详情：
Description
Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry ａnd attachment, condensation of the cylasm ａnd nucleus, ａnd internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, ａnd binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS ａnd thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation.
Components
| Part No. | Description | Size |
| 51-65874X | FITC Annexin V | 0.5 mL (1 ea) |
| 51-66211E | Propidium Iodide Staining Solution | 2 mL (1 ea) |
| 51-66121E | 10X Annexin V Binding Buffer | 50 mL (1 ea) |
PREPARATION AND STORAGE
Store undiluted at 4°C ａnd protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
