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辣根酶化学发光试剂盒,抗兔

辣根酶化学发光试剂盒,抗兔

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简单介绍:辣根酶化学发光试剂盒,抗兔
WESTERN MAX™ CHEMILUMINESCENT HRP KIT, RABBIT

辣根酶化学发光试剂盒,抗兔

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WESTERN MAX™ CHEMILUMINESCENT HRP KIT, RABBIT

辣根酶化学发光试剂盒,抗兔

Description

The detection of proteins in immunoblotting applications is typically achieved by using an antibody that is conjugated to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). The primary antibody which directly binds to the protein target may be labeled with the enzyme, or a labeled secondary antibody may be used to bind to an unlabeled primary antibody. The covalently attached enzyme is capable of degrading a substrate, which may be chromogenic or chemiluminescent. Chromogenic substrates are degraded into colored precipitates and are easy to use. No special equipment is required for detection. Chemiluminescent reactions produce light and are more sensitive than chromogenic reactions, but the image must be recorded on x-ray film or with a gel documentation system. For both types of substrates, the signal generated by the enzyme catalyzed reaction is detected on the membrane only at the site of the target protein, if the immunoblotting conditions are optimized.

AMRESCO's Western Max™ Chemiluminescent Kits contain all the necessary components to obtain sensitive detection of proteins, except for the primary antibody. The kits are compatible with both PVDF and nitrocellulose. Kit includes IgG Positive Control, Dilution Buffer Powder, Tween® 20, BSA, Fraction V, Western Max™ Guide with Protocol. Each kit has enough reagents for forty 10 cm×10 cm immunoblots.

RISK……………………………………………………………………………………………N/A

SAFETY…………………………………………………………………………………………N/A

辣根酶化学发光试剂盒,抗兔订购信息

PRODUCT CODE

SIZE

STORAGE

N223-KIT

KIT

COLD

辣根酶化学发光试剂盒,抗兔

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