Anti-TNF alpha抗体说明书

Catalog Number : RS-2150R

Quantity size : 100ul （dilute with pH 7.4 0.01 M PBS or antibody diluent ）

background:
Tumor Necrosis Factor Alpha （TNF alpha） is a protein secreted by lipopolysaccharide stimulated macrophages, ａnd causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock ａnd tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta （lymphotoxin）, sharing the same receptors ａnd cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts ａnd other cell lines, activating polymorphonuclear neutrophils ａnd osteoclasts, ａnd induction of interleukin 1, prostaglandin E2 ａnd collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers ａnd AIDS Related Complex.Anti-TNF alpha抗体说明书

Specificity : Anti-TNF alpha is a rabbit Polyclonal antibody

specific for TNF alpha of Human,cow ,Mouse, Rat, Dog

- use for western blotting,elisa,immunoprecipitation ａnd immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 17/26 kDa

Application :

– WB=1:100-500

– ELISA=1:500-1000

– IHC-P=1:100-500

– IHC-F=1:100-500

– optimal dilutions/concentrations should be determined by the end user..

– KLH conjugated synthetic peptide derived from human TNF alpha

Anti-TNF alpha抗体说明书

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

原创作者：上海瑞齐生物科技有限公司