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小鼠抗聚组氨酸单克隆抗体
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更新日期:2026/8/7 18:14:45
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相关标签:小鼠抗聚组氨酸单克隆抗体
详细内容
英文名称His tag(2D5)中文名称小鼠抗聚组氨酸单克隆抗体别 名HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.说 明 书0.1ml 0.2ml 产品类型标签抗体 研究领域细胞生物 免疫学 抗体来源Mouse克隆类型Monoclonal克 隆 号2D5交叉反应fusionprotein,HHHHHHtagged产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.性 状Lyophilized or Liquid浓 度1mg/1ml免 疫 原KLH conjugated HHHHHH peptide亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.PubMedPubMed产品介绍background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.性 状Lyophilized or Liquid浓 度1mg/1ml免 疫 原KLH conjugated HHHHHH peptide亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.PubMedPubMed产品介绍background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。
小鼠抗聚组氨酸单克隆抗体


