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仓鼠肾成纤维细胞
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详细内容
| 细胞名称 | BHK-21 [C-13]仓鼠肾成纤维细胞 |
| 描述 | 1961年3月，I.A. Macpherson和M.G.P. Stoker从1日龄的仓鼠建立了BHK-21 (C-13)的亲本。随后84天连续培养，仅中断8天进行冻存，通过单细胞分离建立了13号克隆。这株细胞可用作转染宿主，用于表达包含选择及扩增标记的DNA(如Factor VIII,见ATCC CRL-8544)的载体。 |
| 动物种别 | 仓鼠 |
| 性别 | *** |
| 组织来源 | 正常肾 |
| 形态 | 成纤维细胞 |
| 培养基和添加剂 | MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
| 供应限制 | A。仅供研究之用。 |
| REFERENCE | Gitschier J , et al. Genetic mapping ａnd diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene. Nature 314: 738⁻⁷40, 1985. PubMed: 2986011 Hutz MH , et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 Macpherson I , Stoker M . Polyoma transformation of hamster cell clones－－an investigation of genetic factors affecting cell competence. Virology 16: 147-151, 1962. PubMed: 14468055 Virology 14: 359-370, 1961. Macpherson I . Characteristics of a hamster cell clone transformed by polyoma virus. J. Natl. Cancer Inst. 30: 795-815, 1963. Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697⁻⁷04, 1997. PubMed: 8985401 Yang TT , et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645 Hussain MA , et al. POU domain transcription factor brain 4 confers pancreatic alpha-cell-specific expression of the proglucagon gene through interaction with a novel proximal promoter G1 element. Mol. Cell. Biol. 17: 7186⁻⁷194, 1997. PubMed: 9372951 You M , et al. ch-IAp1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328⁻⁷341, 1997. PubMed: 9372964 Jelachich ML , Lipton HL . Theiler＇s murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327 Schnell MJ , et al. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J. Virol. 70: 2318-2323, 1996. PubMed: 8642658 Chang YE , et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia: Standards Australia; Standards Australia AS ISO 10993.5-2002. Biological evaluation of medical devices－－Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 10993-5:1999. Hay, R. J., Caputo, J. L., ａnd Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. ａnd Vesley, D., (1995) Laboratory Safety: Principles ａnd Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological ａnd Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health ａnd Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
| 中文名称 | 仓鼠肾成纤维细胞 |
