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大鼠骨肉瘤细胞
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详细内容
| 细胞名称 | UMR-106大鼠骨肉瘤细胞 |
| 描述 | 注射放射性同位素磷(32P)诱导产生的可移植性大鼠骨肉瘤克隆建立了UMR-106细胞株。 细胞对PTH，前列腺素及破骨甾体有响应。 对PTH的响应度，UMR-106比相关细胞株UMR-108(ATCC CRL-1663)要高。 蛋白激酶C的活化抑制ATP诱导的胞内钙水平的升高。 起始骨肉瘤和克隆株都是University of Sheffield的T.J. Martin建立的。 |
| 动物种别 | 大鼠 |
| 性别 | *** |
| 组织来源 | 器官: 骨骼 品系: Sprague-Dawley 疾病: 骨肉瘤 |
| 形态 | 上皮细胞 |
| 培养基和添加剂 | DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
| 供应限制 | A。仅供研究之用。 |
| REFERENCE | Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex ａnd transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513 Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977. Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists ａnd biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768 Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521 Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375 Ingleton PM, et al. Alkaline phosphatase in serum ａnd tumour of rats bearing a hormone- responsive transplantable osteogenic sarcoma. Eur. J. Cancer 15: 685-691, 1979. PubMed: 292595 Crawford A, et al. Membranes from a transplantable osteogenic sarcoma responsive to parathyroid hormone ａnd prostaglandins: regulation of adenylate cyclase ａnd of hormone metabolism. J. Endocrinol. 77: 213-224, 1978. PubMed: 275436 Atkins D, Martin TJ. Rat osteogenic sarcoma cells:effects of some prostaglandins, their metabolites ａnd analogues on cyclic AMP production. Prostaglandins 13: 861-871, 1977. PubMed: 194286 Martin TJ, et al. Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260: 436-438, 1976. PubMed: 1062678 Ingleton PM, et al. Radiation induced osteogenic sarcoma in the rat as a model of hormone-responsive differentiated cancer. Lab. Anim. Sci. 27: 748⁻⁷56, 1977. PubMed: 338978 Atkins D, et al. Rat osteogenic sarcoma cells: isolation ａnd effects of hormones on the production of cyclic AMP ａnd cyclic GMP. Endocrinology 101: 555-562, 1977. PubMed: 195798 Crawford A, et al. Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha ａnd thromboxane B2 on cyclic AMP production ａnd adenylate cyclase activity. Biochem. Biophys. Res. Commun. 82: 1195-1201, 1978. PubMed: 212039 Gallinaro BJ, et al. Activation of protein kinase C inhibits ATP-induced [Ca2+]I elevation in rat osteoblastic cells: selective effects on P2Y ａnd P2U signaling pathways. J. Cell. Physiol. 162: 305-314, 1995. PubMed: 7860638 Clin. Orthop. Relat. Res. 140: 247-254, 1979. Manolagas SC, et al. 1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines. J. Biol. Chem. 255: 4414-4417, 1980. PubMed: 6929275 Underwood JC, et al. Structural ａnd functional correlations in parathyroid hormone responsive transplantable osteogenic sarcomas. Eur. J. Cancer 15: 1151-1158, 1979. PubMed: 294355 24392: . . Trans. Biochem. Sic. 6: 239-241, 1978. Partridge NC, et al. Morphological ａnd biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43: 4308-4315, 1983. PubMed: 6575864 Martin TJ, et al. Hormonal influences on bone cells. Methods Enzymol. 145: 324-336, 1987. PubMed: 3474491 Forrest SM, et al. Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone ａnd calcitonin. Calcif. Tissue Int. 37: 51-56, 1985. PubMed: 3922597 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651 |
| 中文名称 | 大鼠骨肉瘤细胞 |
