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小鼠纤维肉瘤细胞WEHI-13VAR细胞

点击次数：152发布时间：2017/5/3

更新日期：2017/5/3 11:27:05

生产地：美洲

产品型号：

简单介绍：WEHI-13VAR, is a variant of WEHI 164 clone 13 which has lost its sensitivity to Tumor Necrosis Factor (TNF) in the absence of actinomycin D.

相关标签：小鼠纤维肉瘤细胞WEHI-13VAR 小鼠纤维肉瘤细胞株 WEHI-13VAR细胞株

详细内容

一、产品信息

Organism	Mus musculus, mouse
Product Format	frozen
Morphology	fibroblast
Culture Properties	mixed, adherent ａnd suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	fibrosarcoma
Strain	BALB/c
Applications	This line provides a stable ａnd highly sensitive bioassay system to detect ａnd measure mouse ａnd human natural ａnd recombinant tumor necrosis factors (TNF alpha ａnd lymphotoxin.
Storage Conditions	liquid nitrogen vapor phase

二、产品特点

Derivation
WEHI-13VAR, is a variant of WEHI 164 clone 13 which has lost its sensitivity to Tumor Necrosis Factor (TNF) in the absence of actinomycin D.

Comments
The WEHI-13VAR cell line was found to maintain its high sensitivity to TNF when the assay was performed in the presence of 500 ng/mL actinomycin D.

This cell line is more sensitive to TNF alpha ａnd lymphotoxin than L929 (ATCC CCL-1) or WEHI 164 (ATCC CRL-1751).

三、使用方法

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove ａnd discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium ａnd cells from Step 1 ａnd spin at approximately 125 x g for 5 to 10 minutes.

Discard supernatant ａnd resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subculture Ratio: 1:4 to 1:6
Medium Renewal: Twice a week.

Note: For more information on enzymatic dissociation ａnd subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions
Temperature: 37.0°C

四、历史

Name of Depositor JA Armstrong

References
Abu-Khabar KS, et al. Type I interferons (IFN-alpha ａnd -beta) suppress cytotoxin (tumor necrosis factor-alpha ａnd lymphotoxin) production by mitogen-stimulated human peripheral blood mononuclear cell. J. Leukocyte Biol. 52: 165-173, 1992. PubMed:1506772

Khabar KS, et al. WEHI-13VAR: a stable ａnd sensitive variant of WEHI 164 clone 13 fibrosarcoma for tumor necrosis factor bioassay. Immunol. Lett. 46: 107-110, 1995. PubMed: 7590904

Derivation	WEHI-13VAR, is a variant of WEHI 164 clone 13 which has lost its sensitivity to Tumor Necrosis Factor (TNF) in the absence of actinomycin D.
Comments	The WEHI-13VAR cell line was found to maintain its high sensitivity to TNF when the assay was performed in the presence of 500 ng/mL actinomycin D. This cell line is more sensitive to TNF alpha ａnd lymphotoxin than L929 (ATCC CCL-1) or WEHI 164 (ATCC CRL-1751).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove ａnd discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium ａnd cells from Step 1 ａnd spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant ａnd resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C. Subculture Ratio: 1:4 to 1:6 Medium Renewal: Twice a week. Note: For more information on enzymatic dissociation ａnd subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37.0°C

Name of Depositor	JA Armstrong
References	Abu-Khabar KS, et al. Type I interferons (IFN-alpha ａnd -beta) suppress cytotoxin (tumor necrosis factor-alpha ａnd lymphotoxin) production by mitogen-stimulated human peripheral blood mononuclear cell. J. Leukocyte Biol. 52: 165-173, 1992. PubMed:1506772 Khabar KS, et al. WEHI-13VAR: a stable ａnd sensitive variant of WEHI 164 clone 13 fibrosarcoma for tumor necrosis factor bioassay. Immunol. Lett. 46: 107-110, 1995. PubMed: 7590904