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上海杏宜生物试剂盒公司试剂盒说明书@台风资讯

点击次数：156 发布时间：2021/9/15 14:30:17

Mouse Peroxisome Proliferator Activated Receptor Alpha

(PPAR-α)ELISA Kit

l For research use only.

l For the quantitative in vitro determination of PPAR-α concentrations in Mouse serum, plasma, culture media or any biological fluid.

l Expiration date：six months .

l Storage： 2-8℃.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PPAR-α. Standards or samples are added to the appropriate Microelisa stripplate wells ａnd combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PPAR-α is added to each Microelisa stripplate well ａnd incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PPAR-α and HRP conjugated PPAR-αantibody will appear blue in color and then turn yellow after the addition of the s solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PPAR-α, You can calculate the concentration of PPAR-α in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

	Materials provided with the kit	48 determinations	96 determinations	Storage
1	User manual	1	1	R.T.
2	Closure plate membrane	2	2	R.T.
3	Sealed bags	1	1	R.T.
4	Microelisa stripplate	1	1	2-8℃
5	Standard：2700 pg/ml	0.5ml×1 bottle	0.5ml×1 bottle	2-8℃
6	Standard diluent	1.5ml×1 bottle	1.5ml×1 bottle	2-8℃
7	HRP-Conjugate reagent	3ml×1 bottle	6ml×1 bottle	2-8℃
8	Sample diluent	3ml×1 bottle	6ml×1 bottle	2-8℃
9	Chromogen Solution A	3ml×1 bottle	6ml×1 bottle	2-8℃
10	Chromogen Solution B	3ml×1 bottle	6ml×1 bottle	2-8℃
11	S Solution	3ml×1 bottle	6ml×1 bottle	2-8℃
12	wash solution	20ml(20X)×1bottle	20ml(30X)×1bottle	2-8℃

Sample preparation

1. Serum preparation

After collection of the whole blood, allow the blood to clot by leaving it undisturbed at room temperature. This usually takes 10-20 minutes. Remove the clot by centrifuging at 2,000-3,000 rpm for 20 minutes. If precipitates appear during reservation, the sample should be centrifugated again.

2. Plasma preparation

Collect the whole blood into tubes with anticoagulant (EDTA or citrate). After incubated at room temperature for 10-20 minutes, tubes are centrifugated for 20 min at 2,000-3,000 rpm. Collect the supernatant carefully as plasma samples. If precipitates appear during reservation, the sample should be centrifugated again.